mcp1 primary antibody  (R&D Systems)

Journal: Frontiers in Pharmacology

Article Title: Ningmitai capsules have anti-inflammatory and pain-relieving effects in the chronic prostatitis/chronic pelvic pain syndrome mouse model through systemic immunity

doi: 10.3389/fphar.2022.949316

Figure Lengend Snippet: Antibody information used in the article.

Article Snippet: The antibodies were as follows: fluorescent primary antibody: MCP1/CCL2 (5H2) Mouse mAb (ZENBIO (220691), 1:100) and CD11b Rabbit pAb (ZENBIO (380675), 1:200).

Techniques:

Journal: Cells

Article Title: Immune Cell-Associated Protein Expression Helps to Predict Survival in Muscle-Invasive Urothelial Bladder Cancer Patients after Radical Cystectomy and Optional Adjuvant Chemotherapy

doi: 10.3390/cells10010159

Figure Lengend Snippet: Univariate Cox’s regression analysis: Association between chemotherapy, considering the immune markers CCL2, CD68, and CD163, and prognosis stratified by the nodal stage.

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with tris-ethylenediaminetetraacetic acid (TE)–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques:

Journal: Cells

Article Title: Immune Cell-Associated Protein Expression Helps to Predict Survival in Muscle-Invasive Urothelial Bladder Cancer Patients after Radical Cystectomy and Optional Adjuvant Chemotherapy

doi: 10.3390/cells10010159

Figure Lengend Snippet: Univariate Cox’s regression analysis: Association between chemotherapy, considering the immune markers CCL2, CD68, and CD163, and prognosis stratified by the tumor stage.

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with tris-ethylenediaminetetraacetic acid (TE)–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques:

Journal: Cells

Article Title: Immune Cell-Associated Protein Expression Helps to Predict Survival in Muscle-Invasive Urothelial Bladder Cancer Patients after Radical Cystectomy and Optional Adjuvant Chemotherapy

doi: 10.3390/cells10010159

Figure Lengend Snippet: Kaplan–Meier analysis: Association of chemotherapy with prognosis (OS, DSS, and RFS) in the N0/N1 + 2 groups stratified by CCL2 expression (>6% or ≤6%). In the N0 group ( A – C ) with CCL2 (>6%), chemotherapy treatment was not associated with OS ( A ) or DSS ( B ) but was associated with shorter RFS ( C ; p = 0.044). In the N1 + 2 group ( D – F ) with CCL2 (≤6%), chemotherapy was not associated with OS ( D ) but was associated with longer DSS ( E ; p = 0.031) and longer RFS (F; p = 0.035).

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with tris-ethylenediaminetetraacetic acid (TE)–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Expressing

Journal: Cells

Article Title: Immune Cell-Associated Protein Expression Helps to Predict Survival in Muscle-Invasive Urothelial Bladder Cancer Patients after Radical Cystectomy and Optional Adjuvant Chemotherapy

doi: 10.3390/cells10010159

Figure Lengend Snippet: Kaplan–Meier analysis: Association of chemotherapy with prognosis (OS, DSS, and RFS) in the pT2/pT3 + 4 groups stratified by CCL2 expression (>6% or ≤6%). In the pT2 group ( A – C ) with CCL2 (>6%), chemotherapy was associated with shorter OS ( A ; p = 0.002), shorter DSS ( B ; p < 0.001), and shorter RFS ( C ; p < 0.001). In the pT3 + 4 group ( D – F ) with CCL2 (≤6%), chemotherapy was not associated with OS ( D ), DSS ( E ), or RFS ( F ).

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with tris-ethylenediaminetetraacetic acid (TE)–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Expressing

Journal: Cells

Article Title: Immune Cell-Associated Protein Expression Helps to Predict Survival in Muscle-Invasive Urothelial Bladder Cancer Patients after Radical Cystectomy and Optional Adjuvant Chemotherapy

doi: 10.3390/cells10010159

Figure Lengend Snippet: Multivariate Cox’s regression analysis: Association between chemotherapy and prognosis stratified by nodal stage and tumor stage (adjusted for the immune markers CCL2, CD68, and CD163).

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with tris-ethylenediaminetetraacetic acid (TE)–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques:

Journal: Cells

Article Title: Immune Cell-Associated Protein Expression Helps to Predict Survival in Muscle-Invasive Urothelial Bladder Cancer Patients after Radical Cystectomy and Optional Adjuvant Chemotherapy

doi: 10.3390/cells10010159

Figure Lengend Snippet: Multivariate Cox’s regression analysis: Association between immunological markers and prognosis stratified by nodal stage and tumor stage (adjusted for chemotherapy and the immune markers CCL2, CD68, and CD163).

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with tris-ethylenediaminetetraacetic acid (TE)–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Marker